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TOSHIBA Medical
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Image Search Results
Journal: Journal of Immunology Research
Article Title: Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma
doi: 10.1155/2016/7828916
Figure Lengend Snippet: Effects of rapamycin on protein expressions of p70S6K/p-p70S6K in ESCC cell lines. (a) Antibodies to p-p70S6K and p70S6K, respectively. (b) Semiquantitative values from three independently repeated experiments, which were statistically analyzed by densitometry using software Image J (NIH, USA), are expressed as means ± SD. ∗∗∗ P < 0.001 compared to untreated cells. GAPDH was used as loading control.
Article Snippet: After EC9706 cells transfected with
Techniques: Software, Control
Journal: Journal of Immunology Research
Article Title: Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma
doi: 10.1155/2016/7828916
Figure Lengend Snippet: Interference efficiency of p70S6K siRNA on mRNA and protein of p70S6K in EC9706 cells. (a) Expression of p70S6K mRNA. (b) Semiquantitative values of p70S6K mRNA normalized to GAPDH mRNA. (c) Expressions of p70S6K/p-p70S6K protein. (d) Semiquantitative values of p-p70S6K/p70S6K protein normalized to GAPDH. Results were from three independently repeated experiments analyzed by using Image J (NIH, USA); data were expressed as mean ± SD. # P < 0.05, ## P < 0.01 for mRNA results, and ∗∗∗ P < 0.001 for protein expression, compared to those of EC9706 cells treated with negative control siRNA.
Article Snippet: After EC9706 cells transfected with
Techniques: Expressing, Negative Control
Journal: Journal of Immunology Research
Article Title: Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma
doi: 10.1155/2016/7828916
Figure Lengend Snippet: Effects of rapamycin on cell proliferation. Cells transfected with p70S6K siRNA or negative control siRNA for 24 hours were treated with rapamycin at different concentrations for 48 hours and cell proliferation was detected with CCK-8 kit. Data pooled from three independent experiments were expressed as mean ± SD. ∗∗ P < 0.01 and ∗∗∗ P < 0.001, compared to cells with negative control siRNA treated with rapamycin at the same concentration.
Article Snippet: After EC9706 cells transfected with
Techniques: Transfection, Negative Control, CCK-8 Assay, Concentration Assay
Journal: Journal of Immunology Research
Article Title: Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma
doi: 10.1155/2016/7828916
Figure Lengend Snippet: Effects of rapamycin on cell cycle phase. EC9706 cells were transfected with p70S6K siRNA or negative control siRNA for 24 hours and then treated with 100 nmol/L rapamycin for 48 hours. Cell cycle phase was analyzed by flow cytometry. ∗ P < 0.05, compared to cells with negative control siRNA.
Article Snippet: After EC9706 cells transfected with
Techniques: Transfection, Negative Control, Flow Cytometry
Journal: Journal of Immunology Research
Article Title: Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma
doi: 10.1155/2016/7828916
Figure Lengend Snippet: Tumor regression observed in EC9706 xenografts treated with different ways and the protein expressions of p70S6K and p-p70S6K in xenografts of each group. (a) Tumor volumes from the xenografts of each group were assessed every other day, and the results were expressed as means ± SE (mm 3 ). The tumor growth of each treated group became slow, in which group treated with p70S6K siRNA combined with rapamycin was the slowest. (b) Tumors from the xenografts treated with different ways for two and a half weeks. (c) The protein expression of p70S6K and p-p70S6K in EC9706 xenografts analyzed by Western blot. (d) Semiquantitative values from three independently repeated experiments, which were statistically analyzed by densitometry using software Image J (NIH, USA), are expressed as means ± SD. ∗ P < 0.05, ∗∗∗ P < 0.001 compared to control group. GAPDH was used as loading control.
Article Snippet: After EC9706 cells transfected with
Techniques: Expressing, Western Blot, Software, Control
Journal: Journal of Immunology Research
Article Title: Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma
doi: 10.1155/2016/7828916
Figure Lengend Snippet: Effects of rapamycin alone or combined with p70S6K siRNA on the growth of ESCC xenografts in nude mice ( n = 5).
Article Snippet: After EC9706 cells transfected with
Techniques: Mouse Assay, Inhibition, Control
Journal: Journal of Immunology Research
Article Title: Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma
doi: 10.1155/2016/7828916
Figure Lengend Snippet: Expression of p-p70S6K in human normal esophageal and ESCC tissues by immunohistochemical analysis (×400). (a) Positive expression of p-p70S6K in ESCC tissues. (b) Moderate positive expression of p-p70S6K in dysplasia tissues of the esophagus (×400). (c) Negative expression of p-p70S6K in normal tissues of the esophagus (×400).
Article Snippet: After EC9706 cells transfected with
Techniques: Expressing, Immunohistochemical staining
Journal: Journal of Immunology Research
Article Title: Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma
doi: 10.1155/2016/7828916
Figure Lengend Snippet: Expressions of p-p70S6K protein in different tissues.
Article Snippet: After EC9706 cells transfected with
Techniques:
Journal: Journal of Immunology Research
Article Title: Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma
doi: 10.1155/2016/7828916
Figure Lengend Snippet: Clinical significance of p-p70S6K protein expression.
Article Snippet: After EC9706 cells transfected with
Techniques: Expressing, Membrane
Journal: Journal of Immunology Research
Article Title: Downregulation of p70S6K Enhances Cell Sensitivity to Rapamycin in Esophageal Squamous Cell Carcinoma
doi: 10.1155/2016/7828916
Figure Lengend Snippet: Correlation of the expression level of p-p70S6K and mTOR in ESCC tissues.
Article Snippet: After EC9706 cells transfected with
Techniques: Expressing
Journal: PLoS ONE
Article Title: Sensitivity of IFN-γ Release Assay to Detect Latent Tuberculosis Infection Is Retained in HIV-Infected Patients but Dependent on HIV/AIDS Progression
doi: 10.1371/journal.pone.0001441
Figure Lengend Snippet: Characteristics of patients included in the cohort, with a detail of those responding and those not responding to ELISPOT PHA positive control.
Article Snippet:
Techniques: Enzyme-linked Immunospot, Control
Journal: PLoS ONE
Article Title: Sensitivity of IFN-γ Release Assay to Detect Latent Tuberculosis Infection Is Retained in HIV-Infected Patients but Dependent on HIV/AIDS Progression
doi: 10.1371/journal.pone.0001441
Figure Lengend Snippet: Analysis of the determinants of positive response to the ESAT6/CFP10 ELISPOT assay and to the TST (n = 247).
Article Snippet:
Techniques: Enzyme-linked Immunospot, Infection